Hormone Testing — Rhythm & Menopause Panels

Aligned Modern Health offers two proprietary salivary hormone panels for women: the Rhythm™ panel tracks estradiol, progesterone, and testosterone across 11 saliva samples over a full 28-day cycle for premenopausal and perimenopausal women; the Menopause Plus panel tests estrone, estradiol, estriol, progesterone, DHEA, cortisol, and melatonin over six days. Both panels are processed by Genova Diagnostics and reviewed by specialist practitioners who design personalised hormone treatment plans based on results.

Aligned Modern Health has built a specialised hormone testing service for women navigating the complexities of hormonal change, with two flagship diagnostic panels that go significantly further than standard blood draws. Both panels use saliva as the collection medium, which captures the bioavailable (unbound) fraction of hormones and reflects tissue-level exposure more accurately than serum testing — an approach favoured by functional medicine practitioners for tracking cyclical patterns and tissue uptake. The Rhythm™ panel is designed for premenopausal and perimenopausal women who are not currently using hormone supplements. Over a 28-day menstrual cycle, 11 saliva samples are collected at defined intervals and tested for estradiol, progesterone, and testosterone. This longitudinal view reveals whether ovulation is occurring, whether the luteal phase is adequately supported by progesterone, and whether androgen levels are balanced relative to estrogens — information that a single mid-cycle blood test cannot capture. The resulting hormone curve is reviewed by one of Aligned Modern Health's hormone specialists, who uses it to guide any necessary interventions including bioidentical hormone replacement therapy (BHRT). The Menopause Plus panel is intended for women in perimenopause or postmenopause. Eight saliva specimens are collected over six days and tested for the full estrogen triad (estrone E1, estradiol E2, and estriol E3), progesterone with the P/E2 ratio, testosterone, DHEA, cortisol (at four time points to capture the diurnal pattern), and melatonin. This comprehensive snapshot allows practitioners to assess adrenal function alongside ovarian hormone output — important because the adrenal glands become the primary source of sex hormones after menopause. Both panels are processed by Genova Diagnostics, a reference laboratory known for its functional medicine testing services. Results are reviewed in consultation with Aligned Modern Health's hormone health practitioners, who integrate findings into a broader clinical picture that may also include thyroid function and metabolic markers. The clinic network's Chicago locations — including Andersonville, Lincoln Square, and Wicker Park — provide convenient access for patients across the North Side, Northwest Side, and broader Chicagoland area seeking advanced hormone evaluation.